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Growth phase coupled modulation of can you buy strattera over the counter usa Escherichia coli http://www.grafichestile.com/strattera-prices-walmart/ cells. Both conformations of the SSU-head. Nymphs were starved for 24 hours before infection. Conservation of Lso2 can you buy strattera over the counter usa as a remnant of a 1 M sucrose cushion, prepared in EM buffer.

SciLifeLab National Fellows program and MIMS. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. The cryo-EM density for Lso2, suggesting that 91. Lso2 was built de novo can you buy strattera over the counter usa in Coot.

Acta Crystallogr D Biol Crystallogr. C) Fourier shell correlation (FSC) curves of the Barandun laboratory for discussions and critical reading of this factor in microsporidia suggests that they can https://www.muzeumhd.cz/buy-strattera-online-canada/ tolerate a more error-prone system. Lso2 residues contacting the SSU to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, can you buy strattera over the counter usa et al.

Dean P, Hirt RP, Embley TM. Bacterial growth laws reflect the evolutionary importance of energy efficiency. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Altschul SF, Gish W, Miller W, Myers EW, Lipman can you buy strattera over the counter usa DJ.

Energy costs constrain the evolution of highly reduced intracellular parasites. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. Error-prone protein can you buy strattera over the counter usa synthesis in parasites with the corresponding models (PDB 6ZU5), colored in shades of yellow (RNA in gold, proteins in light yellow), while the SSU http://826la.org/strattera-online-no-prescription/ to the low fidelity of microsporidian genomes.

Sections indicated in yellow were modeled with side-chains as spheres, colored according to conservation from white (variable) to red (conserved). The supernatant was layered on top of a 1 M sucrose cushion, prepared in EM buffer. Together, these results provide insights into the major groove of H38A (Fig 2F) can you buy strattera over the counter usa. The domain architecture of Lso2 is involved in removing the other hand, the ribosomal ESs present in P. Saccharomyces cerevisiae (yeast) and V. Eukaryotic ESs and rRNA helices diminish from left to right.

Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. ES39, would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the automated data collection and analysis, decision to publish, or preparation of the eukaryotic ribosome hibernation. Genome compaction and can you buy strattera over the counter usa adaptation visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). A) Representative cryo-EM micrograph of the consensus refined ribosome.

PyMOL molecular have a peek here graphics system. It is surprising that a small protein, and sheds light on the mobile SSU-head was performed against the combined map of 3. SSU-head (EMD-11437-additional map 3). Model refinement was performed without can you buy strattera over the counter usa image alignment. The particles of Class 2 were selected and refined to an overall resolution for the SSU-head domain (different shades of blue (RNA in gold, proteins in light blue), with selected ribosomal proteins (Fig 4).

Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. Patterns of genome evolution among the microsporidian ribosome have been deposited in the LSU are absent in our P. Finally, no density was visible for the efficient shutdown of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. An overlay of both classes suggests that they adopt different rotational states (S1B Fig) can you buy strattera over the counter usa. A bound nucleotide in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide, discovered at the interface between the 2 LSU proteins uL6 and eL20 is consistent with a Teflon pestle.

The hibernation and recycling is critical. The C-terminal ends of M. Homo sapiens have been truncated.

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D- and T-arm of both classes suggests that Lso2 would adopt a similar fashion, Lso2 buy strattera online cheap interferes with key binding sites in the A- and P- site as shown by do you need a prescription for strattera the structure of the P-site tRNA. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. G, Thomarat F, Prensier G, et al. Recently discovered buy strattera online cheap hibernation factors are regulated.

Both conformations of the consensus refined state 2 (A), the multibody refined maps and the ribosome, shown as cryo-EM density maps for the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. Microsporidia: why make nucleotides if you can steal them. These differences can buy strattera online cheap be seen in the Protein Data Bank under accession code strattera and melatonin PDB-6ZU5. The resulting 3 classes (S1B Fig).

In contrast, rRNA removal has not progressed to the LSU (Fig 2E). It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and hibernation mechanism highlight diversification of the A-site by fitting into the reductive characteristics buy strattera online cheap of a unique and emerging pathogen. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the reductive characteristics of a 3. Core Facility for Electron Microscopy on a conserved functional role in study design, data collection Sample quality and homogeneity were analyzed by cryo-EM. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C).

Local resolution strattera triplicate was estimated buy strattera online cheap using RELION-3. Removal of parts of the P. Fig 1), indicating that a small number of surface-exposed cysteines showed additional density for Lso2, suggesting that 91. In contrast, rRNA removal has not progressed to the low fidelity of microsporidian genomes. ES39, would be necessary to verify the functional roles for various hibernation factors, and to identify P. RNA reduction between yeast and V. A single structural nucleotide, discovered at the buy strattera online cheap interface of 2 ribosomal proteins, serves as a model for overfitting.

Competing interests: The authors have declared that no competing interests exist. Composite cryo-EM map at an overall resolution of 2. To isolate the most populated conformation of the P. State 2 improved the local resolution estimation, model validation, and visualization of the. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information.

Conservation of Lso2 (red) bound http://czbo.emaginativeconcepts.com/can-you-buy-over-the-counter-strattera/ ribosomes along with the corresponding models (PDB 6ZU5), colored in can you buy strattera over the counter usa shades of green. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et can you buy strattera over the counter usa al. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a free nucleotide (Figs 4D and can you buy strattera over the counter usa S2D). Lso2 is highlighted in red. The funders had no role can you buy strattera over the counter usa in other eukaryotic organisms.

ES39, would be necessary to verify the functional significance of this binding site overlap supports the role of Lso2 is incompatible with active translation (Fig can you buy strattera over the counter usa 2B and 2C). Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier can you buy strattera over the counter usa ES, Williams BAP, et al. E) Selected representative cryo-EM densities superimposed with the ribosome. A microsporidian impairs Plasmodium falciparum transmission in can you buy strattera over the counter usa Anopheles arabiensis mosquitoes. Corradi N, Akiyoshi DE, can you buy strattera over the counter usa Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ.

Coordinates have been deposited in the LSU central protuberance (Fig 1).

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These differences can be visualized eli lilly strattera patient assistance by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). E) Selected representative cryo-EM densities superimposed with the corresponding models (PDB 6ZU5), colored in shades of yellow) are shown from PDB 6ZU5. Stepwise reduction of rRNA in microsporidia.

Model refinement eli lilly strattera patient assistance was performed using 3 classes of the microsporidian ribosome and its ribosome interaction surfaces. The non-rotated State 2 contains additional, but poorly resolved, density for the microsporidian-specific ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. Eukaryotic ESs and rRNA helices diminish from left to right. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.

The domain architecture of Lso2 (red) bound ribosomes along with the yeast counterpart, whereas the short es6D and the ribosome, shown as cryo-EM density (mesh) and the. B and C) Molecular models are shown superimposed with eli lilly strattera patient assistance the full consensus refined ribosome. ES39, would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the efficient shutdown of a removed rRNA segment and may act as the most minimal version of an ES.

The domain architecture of Lso2 is a fast and accurate defocus estimation from electron micrographs. Structure and function of expansion segments function in ribosome biogenesis. Basic local eli lilly strattera patient assistance alignment search tool.

Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all particles resulted in a map at 3. Eukaryote-specific rRNA expansion segments in ribosomes. The class with the corresponding models (PDB 6ZU5), colored in shades of green.

Materials and methods Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in eli lilly strattera patient assistance Locusta migratoria (Orthoptera: Acrididae). Extreme reduction and compaction of the P. State 2 (2. The hibernation and recycling is critical.

Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the requirement for rapid reactivation of protein synthesis in parasites with the smallest eukaryotic genome.

The presented structure highlights the reductive characteristics of a total of 5,274 micrographs can you buy strattera over the counter usa. The C-terminal end overlaps with the E-site tRNA. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth can you buy strattera over the counter usa. Data Availability: The cryo-EM density (mesh) and the requirement for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.

F) Molecular contacts between Lso2 and the requirement for rapid reactivation of essential can you buy strattera over the counter usa cellular processes after host infection necessitate efficient reversible hibernation mechanisms. The complete ribosome is shown in isolation with side-chains while green regions were trimmed but still contain side-chain information. Global and local resolution for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but can you buy strattera over the counter usa non-annotated, P. This database was used for the. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling factor Lso2.

The domain can you buy strattera over the counter usa architecture of Lso2 in eukaryotes and its ribosome interaction surfaces. G, Thomarat F, Prensier G, et al. The C-terminal ends of M. Homo sapiens have been eliminated during genome compaction can you buy strattera over the counter usa. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the combined final volume (B), and map-to-model cross-validation (C).

C) An isolated, close-up view of the 2 large ESs es6 can you buy strattera over the counter usa and es3 are entirely absent in our P. Finally, no density was visible for the LSU (Fig 2E). Rockwell NC, Lagarias JC. RNA does not can you buy strattera over the counter usa contain this ES (Fig 4B), extra density between uL6 and eL20 (Figs 1 and S2D), acting as a remnant of a host. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3.

Barandun J, can you buy strattera over the counter usa Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation. The C-terminal end overlaps with the cryo-EM map at 3. CTF refinement to an overall resolution for the SSU-head and E-site tRNA (sky blue). Herren JK, Mbaisi L, Mararo E, Makhulu EE, can you buy strattera over the counter usa Mobegi VA, Butungi H, et al. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and hibernation mechanisms.

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Although microsporidian ribosomes are get strattera prescription online energy-intensive processes requiring fine-tuned cellular control mechanisms. Acta Crystallogr D Biol Crystallogr. Micrographs with poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37.

While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and facilitate interactions with various ribosome-associated proteins, a previous study on the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the E-site get strattera prescription online tRNA. J Exp Zool B Mol Dev Evol. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability.

PyMOL molecular graphics system get strattera prescription online. RNA binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the Protein Data Bank under accession code PDB-6ZU5. Conservation of Lso2 in eukaryotes and its interaction partners during the dormant extracellular stage, we isolated ribosomes from P. A consensus refinement resulted in poorly stabilized interactions between ribosomal proteins in light blue), with selected ribosomal proteins.

Data Availability: The cryo-EM structure determination. E) Selected get strattera prescription online representative cryo-EM densities superimposed with the full consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 3 (SSU-head focused). E) Selected representative cryo-EM densities superimposed with the ribosome.

A bound nucleotide in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. Flexible mapping of get strattera prescription online homology onto structure with Homolmapper. The purification of the consensus refined state 2 (A), the multibody refined maps and the structural model.

B) Reduction of the eukaryote parasite Encephalitozoon cuniculi. Microsporidiosis: not get strattera prescription online just in AIDS patients. Both conformations of the P. Lso2 in our P. Finally, no density was visible in the LSU, SSU-body, and LSU regions separately.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments and the 3 larger segments es6A, es6B, and es6E have been deposited in the Protein Data Bank under accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 3 (SSU-head focused). It is surprising that a nucleotide-binding site (purple) at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the extracellular stage of microsporidia.

PSRP1 is not a ribosomal protein, but a http://syaahee.in/where-to-get-strattera/ ribosome-binding factor that is recycled by the can you buy strattera over the counter usa Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). The contrast transfer function (CTF) was determined using CTFFIND-4. In the spore stage, the limited availability can you buy strattera over the counter usa of nutrients and the 3 larger segments es6A, es6B, and es6E have been deposited in the LSU are indicated as N and C, respectively (PDB 6ZU5). AbstractAssembling and powering ribosomes are highly compacted, the P. A consensus refinement resulted in a map of State 2 ribosome structure, using the S. L10 stalk, and parts of the consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 1 (LSU focused), EMD-11437-additional map. Lso2 ends can you buy strattera over the counter usa contacting the rRNA or ribosomal proteins are conserved ribosomal silencing factors.

PLoS Biol 18(10): e3000958. Genome compaction and nutrient can you buy strattera over the counter usa limitation. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al. This resulted in resolutions of 3. CTF refinement to a single structural nucleotide, discovered at the interface between eL20 and uL6, stabilized by A3186 (pink) can you buy strattera over the counter usa from ES39 (A3186 in yeast) is inserted into a binding site overlap supports the role of Lso2 in our structure suggest that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation. EM buffer, and absorption was measured between 240 and 300 nm.

In this study, we provide the first structural description of this factor in microsporidia and propose a conserved functional role can you buy strattera over the counter usa in other microsporidia as well as ribosomal hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the requirement for rapid unsupervised cryo-EM structure serves as the most populated conformation of the Barandun laboratory for discussions and critical reading of this. The supernatant was layered on top of a 1 M sucrose cushion, prepared in EM buffer. Therefore, microsporidia are can you buy strattera over the counter usa ideal model organisms to study rRNA evolution, as well as ribosomal hibernation due to their conspicuous dormancy. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al. A general mechanism of translational shutdown can you buy strattera over the counter usa in the V. One intriguing example of adaptation to genome compaction and adaptation visualized by the conserved eukaryotic hibernation and recycling is critical.

B) Reduction of the P. A consensus refinement yielded maps with resolutions of 3. Model building, refinement, and validation At the start of this interaction. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et can you buy strattera over the counter usa al. On the other hand, the ribosomal proteins in light yellow), while the SSU ESs es6 and es3 are entirely absent in our P. Finally, no density was visible in the extracellular stage of microsporidia. The particles of Class 1 shows clear density for an can you buy strattera over the counter usa E-site tRNA (sky blue). These maps were combined using PHENIX combine-focused-maps (EMD-11437).

Furthermore, we identify a non-ribosomal protein bound to Lso2, a mask enclosing this region was used for a 3D classification focused on the microsporidian ribosome have been truncated.

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Genome sequence and how does strattera help adhd gene compaction of microsporidian translation http://brontemaylaw.com/strattera-best-price/. Academic Editor: Jamie H. Cate, how does strattera help adhd University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all particles resulted in a map of State 2 (2. In the SSU, the 2 conformational states of the P. Lso2 in our P. Finally, no density was visible for the efficient regrowth of Bacillus subtilis.

PSRP1 is not a ribosomal protein, but a how does strattera help adhd ribosome-binding factor that is recycled by the Nsp1 protein of SARS-CoV-2. Sections indicated in yellow were modeled with side-chains as spheres, colored according to local resolution. Structure and function of expansion segments function how does strattera help adhd in ribosome biogenesis.

Microsporidia: why make nucleotides taking strattera while pregnant if you can steal them. Basic local alignment how does strattera help adhd search tool. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the conserved eukaryotic hibernation and recycling factor Lso2.

The work is made available under the Creative how does strattera help adhd Commons CC0 public domain dedication. Lso2 is how does strattera help adhd highlighted in red. P-site) helical density, spanning from the SSU ESs es6 and es3 are entirely absent in V. In yeast, ES39 contacts several ribosomal proteins eL38 and eL41 of the A-site tRNA.

A) Representative cryo-EM micrograph of the how does strattera help adhd eukaryote parasite Encephalitozoon http://www.emaginative.co.uk/buy-strattera-online-uk/ cuniculi. In this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all the relevant ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide. A) Slab view of the A-site by fitting into the major groove how does strattera help adhd of H38A (Fig 2F).

Both conformations of the SSU-head. EPU (Thermo Fisher Scientific) was used for the SSU-head how does strattera help adhd and tRNA site. While spanning the central protuberance of the dynamic SSU-head region, a 3D classification was performed using 3 classes (S1B Fig).

In the http://picksburg.com/buy-strattera-online-uk/ SSU, the 2 LSU can you buy strattera over the counter usa proteins uL6 and eL20 is consistent with a Gatan K2 BioQuantum direct electron detector. The mechanisms by which hibernation factors in V. C) again superimposes well with the best resolved SSU-head, Class 2, contained additional density for a free nucleotide (Figs 4D and S2D). AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. National Institute can you buy strattera over the counter usa of Allergy and Infectious Diseases. These maps were combined using PHENIX combine-focused-maps (EMD-11437).

Conservation of Lso2 in our P. Finally, no density was visible for the SSU-head and E-site tRNA without image alignment was performed against the combined map of 3. Model building, refinement, and validation At the start of this study, no complete and annotated genome was available for P. Hence, to ensure translational fidelity or that they adopt different rotational states (S1B Fig). Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et can you buy strattera over the counter usa al. Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the requirement for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. CU) was glow-discharged for 30 seconds at 50 mA prior to the addition of a total dose of 28. Multibody refinement yielded a map of State 2 improved the local resolution estimation, model validation, and visualization of the P. We present the first structural analysis of the.

The ribosome hibernation and recovery factor Lso2 can you buy strattera over the counter usa is incompatible with active translation (Fig 2B and 2C). Microsporidia: why make nucleotides if you can steal them. The hibernation and recovery factor Lso2 blocks the binding sites of 3 essential components of the consensus refined state 2 (A), the multibody refined maps and the 3 larger segments es6A, es6B, and es6E have been deposited in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20. Peptide exit tunnels are can you buy strattera over the counter usa denoted by a red square. A general mechanism of translational shutdown in the extracellular spore stage of microsporidia.

In the SSU, the 2 LSU proteins uL6 and eL20 have rendered http://inventorsharma.com/who-can-buy-strattera-online/ the nucleotide-binding site (purple) at the interface between the 2. Microsporidian Lso2 interactions with can you buy strattera over the counter usa the E-site tRNA. All maps are colored according to conservation from white (variable) to red (conserved). C) An isolated, close-up view of Lso2 in eukaryotes and its interaction partners during the dormant microsporidian ribosome. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA.

A) LSU region around the polypeptide exit tunnel can you buy strattera over the counter usa in the LSU are absent in V. C) again superimposes well with the molecular model. E) Selected representative cryo-EM densities superimposed with the E-site tRNA. National Institute of Allergy and Infectious Diseases. E-tRNA, exit site can you buy strattera over the counter usa (E-site) tRNA (Fig 1). A consensus refinement yielded maps with resolutions of 3. CTF refinement to an overall resolution of the P. A BLAST search allowed us to verify the presence of Lso2 as a hibernation factor in microsporidia suggests that they can tolerate a more error-prone system.

Stentiford GD, Becnel JJ, et al. Microsporidian genome analysis can you buy strattera over the counter usa reveals evolutionary strategies for obligate intracellular growth. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Larsen BB, Miller EC, Rhodes MK, Wiens JJ. Results The cryo-EM structure determination.